A recently published study, “Soil eDNA-based droplet digital PCR assay as a new monitoring tool for terrestrial mammals”, led by a group from the Institute for Biological Research “Siniša Stanković” in Belgrade, Serbia, under the guidance of Prof. Vanja Bugarski-Stanojević and driven by the work of first author Marko Đokić, demonstrates the effectiveness of QX200 droplet digital PCR from Bio-Rad Laboratories for identifying vertebrate species from soil-derived environmental DNA (eDNA).

Key highlights:

• Validates ddPCR as a tool for identifying vertebrate taxa from soil eDNA
• Protocol optimized for identifying five cryptic species of the lesser blind mole rat, targeting the cytochrome b
• Provides a framework for conservation strategies focused on elusive or endangered species
• Adaptable for broader application to other terrestrial vertebrates of concern

Labena contributed to this project through:

• Development of species-specific primers and probes for ddPCR-based assays
• Hosting a two-day hands-on workshop on ddPCR laboratory techniques
• Contribution to the publication through co-authorship by Labena’s Lucija Markulin

 

This collaboration showcases how precision molecular tools like ddPCR are transforming conservation science by making species detection faster, more sensitive, and non-invasive.