Depending on the specific goals of the project and/or experiment, the eDNA Labs team performs environmental DNA analysis from sample collection to bioinformatic analysis.

At eDNA Labs we are able to detect selected species using PCR methods (ddPCR or qPCR) and assess the composition of entire communities using next-generation sequencing methods. From project planning to delivery of results, the eDNA Labs service is tailored to your needs with professional support provided in all steps of the process.

SAMPLING SERVICES

• eDNA extraction

TARGET SPECIES DETECTION

Species-specific protocols developed for qPCR or ddPCR for the detection of:

• Invasive species
• Bioindicator species
  • Brown trout  (Salmo trutta)
  • European Grayling (Thymallus thymallus)
  • Huchen (Hucho hucho)
  • Schneider (Alburnoides bipunctatus)
  • Common Chub (Squalius cephalus)
  • Pike (Esox lucius)
  • Common Barbel (Barbus barbus)
  • Tench (Tinca tinca)
  • Common Carp (Cyprinus carpio)
• Specific species based on your interest

BIODIVERSITY MONITORING

SEQUENCING SERVICES

BIOINFORMATIC SERVICES

• Metabarcoding pipelines (12S rRNA, 16S rRNA, rbcL)
• Raw reads filtering
• Pair-end read merging
• Dereplicating
• Error removal (denoising/clustering)
• Chimera sequences removal
• Taxonomical classification

BARCODING SERVICES  (SPECIES IDENTIFICATION)

Taxonomical identification of unknown specimen (DNA extraction, PCR amplification, Sanger sequencing).

ASSAY DEVELOPMENT

Tailor made assay design based on your needs from in silico assay design, primers and probe design, assay optimization and validation to samples analysis.

Need more info on our services or have additional questions? Feel free to contact us at ednalabs@labena.hr