eDNA workshop at IBISS

In October, members of eDNALabs, Teja Muha and Lucija Markulin, visited the Institute for Biological Research “Siniša Stanković” (IBISS), a leading multidisciplinary scientific institution in Serbia specializing in biological sciences. Hosted by Stoimir Kolarević, the main organizer of the workshop, and other members of the IBISS team, eDNALabs team members held an intensive three-day workshop on environmental DNA analysis, covering everything from field sampling to data analysis.

Workshop started with water sampling and filtration on the first day. Sampling activities were done at River Gradac near Valjevo, where both teams collected water samples for eDNA analysis.  IBISS team also performed conventional monitoring using electrofishing method at the same time to be able to correlate traditional monitoring data with results of eDNA molecular analysis.

Following day was dedicated to laboratory work, where Teja Muha and Lucija Markulin guided the participants through the steps involved in eDNA extraction and preparation of reactions for species-specific detection using ddPCR. For this practical demonstration, assays developed by the eDNALabs team were used, showcasing the technology’s potential for identifying Thymallus thymallus (European Grayling) and Salmo trutta (brown trout) in the water samples.

Visit and workshop were concluded with final analysis and comparison of the results of ddPCR assays and the outcomes of the electrofishing activities, followed by the presentation on ongoing eDNA Labs projects and ideas for future collaborations.

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