{"id":2291,"date":"2025-11-19T11:40:15","date_gmt":"2025-11-19T10:40:15","guid":{"rendered":"https:\/\/ednalaboratories.com\/?p=2291"},"modified":"2025-11-19T11:51:10","modified_gmt":"2025-11-19T10:51:10","slug":"soil-dna-meets-cutting-edge-tech-a-breakthrough-in-species-detection","status":"publish","type":"post","link":"https:\/\/ednalaboratories.com\/en\/soil-dna-meets-cutting-edge-tech-a-breakthrough-in-species-detection\/","title":{"rendered":"Soil DNA meets cutting-edge tech: a breakthrough in species detection!"},"content":{"rendered":"<p>A recently published study, \u201c<strong><em>Soil eDNA-based droplet digital PCR assay as a new monitoring tool for terrestrial mammals<\/em><\/strong>\u201d, led by a group from the Institute for Biological Research &#8220;Sini\u0161a Stankovi\u0107&#8221; in Belgrade, Serbia, under the guidance of Prof. <strong>Vanja Bugarski-Stanojevi\u0107<\/strong> and driven by the work of first author <strong>Marko<\/strong> <strong>\u0110oki\u0107<\/strong>, demonstrates the effectiveness of <strong>QX200 droplet digital PCR<\/strong> from <strong>Bio-Rad Laboratories<\/strong> for identifying vertebrate species from <strong>soil-derived environmental DNA<\/strong> (<strong>eDNA<\/strong>).<\/p>\n<p>Key highlights:<\/p>\n<ul>\n<li>Validates ddPCR as a tool for identifying vertebrate taxa from soil eDNA<\/li>\n<li>Protocol optimized for identifying five cryptic species of the lesser blind mole rat, targeting the <em>cytochrome b<\/em><\/li>\n<li>Provides a framework for conservation strategies focused on elusive or endangered species<\/li>\n<li>Adaptable for broader application to other terrestrial vertebrates of concern<\/li>\n<\/ul>\n<p>Labena contributed to this project through:<\/p>\n<ul>\n<li>Development of species-specific primers and probes for ddPCR-based assays<\/li>\n<li>Hosting a two-day hands-on workshop on ddPCR laboratory techniques<\/li>\n<li>Contribution to the publication through co-authorship by Labena\u2019s Lucija Markulin<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<p>This collaboration showcases how precision molecular tools like ddPCR are transforming conservation science by making species detection faster, more sensitive, and non-invasive.<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p><a href=\"https:\/\/www.sciencedirect.com\/science\/article\/pii\/S2351989425003816\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-medium wp-image-2278\" src=\"https:\/\/ednalaboratories.com\/wp-content\/uploads\/2025\/10\/Edna-RAD-293x300.png\" alt=\"\" width=\"293\" height=\"300\" srcset=\"https:\/\/ednalaboratories.com\/wp-content\/uploads\/2025\/10\/Edna-RAD-293x300.png 293w, https:\/\/ednalaboratories.com\/wp-content\/uploads\/2025\/10\/Edna-RAD-626x640.png 626w, https:\/\/ednalaboratories.com\/wp-content\/uploads\/2025\/10\/Edna-RAD-147x150.png 147w, https:\/\/ednalaboratories.com\/wp-content\/uploads\/2025\/10\/Edna-RAD-176x180.png 176w, https:\/\/ednalaboratories.com\/wp-content\/uploads\/2025\/10\/Edna-RAD-360x368.png 360w, https:\/\/ednalaboratories.com\/wp-content\/uploads\/2025\/10\/Edna-RAD.png 761w\" sizes=\"auto, (max-width: 293px) 100vw, 293px\" \/><\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>A recently published study, \u201cSoil eDNA-based droplet digital PCR assay as a new monitoring tool for terrestrial mammals\u201d, led by a group from the Institute for Biological Research &#8220;Sini\u0161a Stankovi\u0107&#8221; in Belgrade, Serbia, under the guidance of Prof. Vanja Bugarski-Stanojevi\u0107 and driven by the work of first author Marko \u0110oki\u0107, demonstrates the effectiveness of QX200 [&hellip;]<\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":true,"template":"","format":"standard","meta":{"footnotes":""},"categories":[72],"tags":[],"class_list":["post-2291","post","type-post","status-publish","format-standard","hentry","category-novost"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v26.3 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Soil DNA meets cutting-edge tech: a breakthrough in species detection! 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